ABSTRACT
El objetivo del presente trabajo fue evaluar la respuesta de la aplicación doble o triple de prostaglandina sobre la regresión de CL, ovulación y retorno al celo después de un tratamiento superovulatorio en vacas donantes de embriones. Para este experimento se tomaron 44 vacas asignadas al azar a dos grupos de tratamiento. Al primero (n = 23; CLO 0 & 7) se le aplicó una dosis de 500 μg de cloprostenol los días 0 (denominado día de la colecta) y 7; al segundo grupo (n = 21; CLO 0, 1 & 7) se le aplicó una inyección adicional el día 1. Los animales fueron examinados por ultrasonografía los días 0, 4, 7, 10 y 13 para evaluar la regresión de cuerpos lúteos y la ovulación. Se realizó observación del celo dos veces al día (mañana y tarde). El 60,8 y 52,3% de los grupos CLO 0 & 7 y CLO 0, 1 & 7, respectivamente, presentaron signos de celo. No se presentaron diferencias en el día del celo entre los grupos (7,2 ± 0,3, 7,3 ± 0,5 días). Tampoco hubo diferencia para la variable ovulación (8,5 ± 0,9 y 9,8± 0,9 días). Todas las vacas tratadas regresaron los cuerpos lúteos antes de finalizar el experimento. Cuando se analizó el tiempo de ovulación, se encontró que las vacas con 4 o menos cuerpos lúteos ovulan en un tiempo menor (P < 0,01) que las vacas con 5 o más cuerpos lúteos. Se concluye que no hay diferencia entre la doble o triple aplicación de cloprostenol para las variables. Sería innecesaria una aplicación adicional de cloprostenol el día 7.
The purpose of the present study was to evaluate the effect of prostaglandin (PG) treatment schedule on luteal regression and return to estrus in superovulated cows. A group of 44 donor cows were randomly assigned to two groups on the day of ova/ embryo collection (day 0). The first group (n = 23; CLO 0 & 7) was treated with 500 μg of cloprostenol on days 0 and 7 after embryo recovery; the second group (n = 21; CLO 0, 1 & 7) received an additional treatment with cloprostenol on day 1. All animals were scanned by transrectal ultrasonography on days 0 (embryo recovery), 4, 7, 10 and 13 to evaluate CL regression and subsequent ovulation. Estrus detection was done twice daily (am/pm) starting on day 4. There were no significant differences between groups in the percentage of animals returning to estrus (60.8% and 52.3%, respectively), or the intervals from the first PG treatment to estrus (7.2 ± 0.3 vs 7.3 ± 0.5 days), or ovulation (8.5 ± 0.9 vs 9.8± 0.9 days). All cows had luteolysis before the end of the experiment. Animals that had 4 CL or less ovulated earlier than cows that had 5 or more CL (P<0.01). In summary, there was no difference in luteal regression and the return to estrus and ovulation in donor cows treated with cloprostenol twice or three times after superovulation. It would not appear to be necessary to treat donors with PG 7 days later, since all cows had luteal regression after the first PG treatment.
ABSTRACT
Fifty-five patients with chronic viral hepatitis B were randomly divided into two groups. Thirty patients were treated with Phyllanthus amarus compound (PA Co) for three months in the treatment group, another 25 patients were treated with domestic recombinant human interferon alpha-1b (IFN-alpha 1b) for three months as controls. The total effective rate in the treatment group was 83.3%, showing no significant difference from the control (p>0.05). The normalization rates of ALT, A/G and SB in the treatment group were 73.3%, 80.0% and 78.2% respectively, which were significantly higher than that in the control (p<0.05). The negative conversion rates of HBeAg and HBV-DNA in the treatment group were 42.3% and 47.8%, showing no significant difference from the control (p>0.005). It is indicated that PA Co has remarkable effect for chronic viral hepatitis B in recovery of liver function and inhibition of the replication of HBV.